In 2022, SLELO PRISM and APIPP are collaborating to continue environmental DNA sampling that has been occurring in SLELO PRISM and expand into the Adirondack’s region. The results from this project will help to improve our understanding of aquatic invasive species distribution and enhance our aquatic early detection work. Sampling sites are selected based on the most highly probable areas to encounter new invasive species introductions or experience the spread of invasive species that are present in other regions. Rather than conducting transect based sampling, we will focus our efforts in areas and habitats that these invasive species would most likely be found. An initial assessment was conducted using satellite imagery and GIS tools to select sites which was followed by field verification. This allowed us to be the most cost-effective and efficient with our time and efforts. Sampling protocol follows the 2021 QAQC developed by SLELO PRISM and The Nature Conservancy Tributary Project. A total of 66 sampling sites have been selected. All amplifications of high-level species indicating potential presence will be reported to NYSDEC where continued communication on response and next steps will be planned collaboratively. The project will be summarized in a report at the end of the field season, which may include sample results, watershed summaries,  and recommendations for potential future projects.